Multicolour localization microscopy by point-spread-function engineering.

3-D PSF fitting for fluorescence microscopy: implementation and localization application.

Localization microscopy relies on computationally efficient Gaussian approximations of the point spread function for the calculation of fluorophore positions. Theoretical predictions show that under specific experimental conditions, localization accuracy is significantly improved when the localization is performed using a more realistic model. Here, we show how this can be achieved by consideri...

Optimal 3D single-molecule localization for superresolution microscopy with aberrations and engineered point spread functions.

Photo-activation localization microscopy is a far-field superresolution imaging technique based on the localization of single molecules with subdiffraction limit precision. Known under acronyms such as PALM (photo-activated localization microscopy) or STORM (stochastic optical reconstruction microscopy), these techniques achieve superresolution by allowing only a sparse, random set of molecules...

Dispersion, aberration and deconvolution in multi-wavelength fluorescence images.

The wavelength dependence of the incoherent point spread function in a wide-field microscope was investigated experimentally. Dispersion in the sample and optics can lead to significant changes in the point spread function as wavelength is varied over the range commonly used in fluorescence microscopy. For a given sample, optical conditions can generally be optimized to produce a point spread f...

Algorithm for 3d Localization Microscopy Using Realistic Modeling of the Point Spread Function by Phase Retrieval

Extraction of Depth Information in the Presence of Spherical Aberration Using Double-helix Point Spread Function Coding in 3d Fluorescence Microscopy Imaging

ABERRATION USING DOUBLE-HELIX POINT SPREAD FUNCTION CODING IN 3D FLUORESCENCE MICROSCOPY IMAGING Chrysanthe Preza, Sreya Ghosh, Ginni Grover, Sean Quirin, and Rafael Piestun Dept. of Electrical & Computer Eng., 206 Engineering Science Bldg., The University of Memphis, Memphis, TN 38152-3180, USA Dept. of Electrical and Computer Engineering, University of Colorado at Boulder, Boulder, Colorado 8...